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KMID : 0363620080290020151
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Journal of Korean Oriental Medicine
2008 Volume.29 No. 2 p.151 ~ p.164
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A Study on the Inhibitory Effect of Yeongdamsagantang on Alzheimer in AB-oligomer-induced Neuro 2A Cell Lines
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Kim Hae-Su
Shin Yoo-Jung
Park Chi-Sang
Park Jong-Hyuk
Kim Seong-Mo
Baek Kyung-Min
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Abstract
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Objective: To investigate the effects of Yeongdamsagantang (YDGT) on apoptosis of neuronal cells that can result
in dementia.
Method: The water extract of the YDGT was tested in vitro for its beneficial effects on neuronal survival and
neuroprotective functions, particularly in connection with Aβ oligomer-related dementias. Aβ oligomers derived from
proteolytic processing of the β-amyloid precursor protein (APP), including the amyloid-β peptide (Aβ), play a critica
role in the pathogenesis of Alzheimer’s disease. A neuroblastoma cell line stably expressing an Aβ oligomerassociated
neuronal degeneration was used to investigate if YDGT inhibits formation of Aβ oligomer. To measure the ATP generating level in mitochondrial membrane, luciferin/luciferase luminescence kit (Promega) and luminator was used, and to survey the protein’s apparition, confocal microscopy was used.
Result: Aβoligomer had a profound attenuation in the increase in CT105 expressing neuro2A cells from YDGT.
Experimental evidence indicates that YDGT protected against neuronal damage from cells, but its cellular and molecular mechanisms remain unknown. We demonstrated that YDGT inhibited formation of amyloid-β (Aβ) oligomers, which were the behavior, and possibly causative, features of AD. The decreased Aβ oligomer in the presence of YDGT was observed in the conditioned medium of this Aβoligomer-secreting cell line under in vitro. In the cells, YDGT significantly attenuated mitochondrion-initiated apoptosis.
Conclusion: (i) a direct Aβ oligomer toxicity and the apoptosis initiated by the mitochondria; and (ii) multiple cellular and molecular neuroprotective mechanisms, including attenuation of apoptosis and direct inhibition of Aβ oligomer
aggregation, underlie the neuroprotective effects of YDGT.
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KEYWORD
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Yeongdamsagantang (YDGT), inhibition of, oligomer aggregation, Alzheimer
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